Abstract: This study aims to gain a baseline of information about basic water quality conditions and pathogens along the Toms River, New Jersey. The main focus of the project is the identification of specific areas, “hotspots” that may be sources of pathogenic bacteria that have a negative impact on water quality at the Toms River and consequently Barnegat Bay. Data was collected by the Save Barnegat Bay water quality team from June 8 to July 29, 2020 from 12 sites along the Toms River from Ocean Gate to Island Heights. One site, Jeffrey’s Creek in Ocean Gate (site A-1) was a semi-enclosed body of water with a high density of waterfowl used as a baseline indicator for pathogenic bacteria (E. coli). Jeffrey’s Creek (A-1), Beachwood Beach (A-4), Dillon’s Creek (B-10) reported an average test above the bacterial threshold of 200 colonies/100 mL for E. coli using the Coliscan EasyGel Method. Additionally, the water quality parameters of the northern part of the river varied, as a whole, from the southern areas which may have due to prevalent south to north winds during the summer season.